A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR-Reference-Cited by-同舟云学术

A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR

Published:2021-11-27 Issue:4 Volume:46 Page:
ISSN:0250-5991
Container-title:Journal of Biosciences
language:en
Short-container-title:J Biosci

Author:

Mote Ridim D,Laxmikant V Shinde,Singh Surya Bansi,Tiwari Mahak,Singh Hemant,Srivastava Juhi,Tripathi Vidisha,Seshadri Vasudevan,Majumdar Amitabha,Subramanyam Deepa^ORCID

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Biochemistry, Genetics and Molecular Biology,General Medicine

Link

https://link.springer.com/content/pdf/10.1007/s12038-021-00231-w.pdf

Reference17 articles.

1. Boyer LA, Lee TI, Cole MF, et al. 2005 Core transcriptional regulatory circuitry in human embryonic stem cells. Cell 122 947–956

2. Dhami MK and Kumarasinghe L 2014 A HRM Real-Time PCR assay for rapid and specific identification of the emerging pest spotted-wing Drosophila (Drosophila suzukii). PLOS ONE 9 e98934

3. Eischeid AC 2011 SYTO dyes and EvaGreen outperform SYBR Green in real-time PCR. BMC Res. Notes 4 263

4. Graham TGW, Dugast-Darzacq C, Dailey GM, Darzacq X and Tjian R 2021 Simple, inexpensive RNA isolation and one-step RT-qPCR methods for SARS-CoV-2 detection and general use. Curr. Protoc. 1 e130

5. Green MR and Sambrook J 2018 2018 Quantification of RNA by real-time reverse transcription-polymerase chain reaction (RT-PCR). Cold Spring Harb. Protoc.

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