Bioactive compounds and antioxidant activity in various parts of Morus alba L. Cv. ichinose: a comparative analysis

Abstract

AbstractPresent investigation was undertaken to assess the composition of bioactive compounds within various parts of methanolic extracts from mulberry plants utilizing both High-Performance Liquid Chromatography (HPLC) and spectrophotometric methodologies. Furthermore, the antioxidant potential was evaluated through assays including 2,2-diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), β-carotene bleaching (BCBA), and Hydroxyl Radical Scavenging Activity (HRSA). This research incorporated the analysis of the fruit, root, shoot, and leaf of the mulberry (Morus alba L.) Cv. Ichinose. Findings from the investigation revealed that levels of total phenols (533.8 ± 15.6 mg/100 g) and flavonoids (232.3 ± 7.1 mg/100 g) were notably higher in fruit compared to root, while total ascorbic acid content (70.9 ± 3.1 mg/100 g) was significantly elevated in the leaf. The fruit exhibited a significantly higher concentration of total anthocyanins (142.5 ± 3.1 mg/100 g), whereas the leaf contained a substantial amount of total carotenoids (5.8 ± 0.3 mg/100 g). Predominant phenolic acids detected in mulberry included ferulic acid, m-coumaric acid, syringic acid, caffeic acid, chlorogenic acid, and gallic acid. Quercetin and isoquercetin were identified as the major flavonoids in mulberry fruits. In terms of anthocyanins, cyanidin-3-rutinoside (25.6 ± 2.3 mg/100 g) and cyanidin-3-glucoside (16.4 ± 2.1 mg/100 g) were the primary anthocyanins present in the fruit. The main carotenoid found in the leaf was beta-carotene, with levels reaching 2.1 ± 0.3 mg/100 g. Fruit displayed the highest antioxidant activity among all parts examined. Comparison of the EC50 values of fruit (EC50 = 174.2 ± 2.2 µg/mL), root (EC50 = 179.6 ± 2.3 µg/mL), and shoot (EC50 = 189.4 ± 2.1) suggested that these segments of the mulberry plant could potentially function as antioxidants and could exhibit a greater hydroxyl radical scavenging effect than standard antioxidants.