Functional approaches to the study of G-protein-coupled receptors in postmortem brain tissue: [35S]GTPγS binding assays combined with immunoprecipitation
Author:
Funder
Eusko Jaurlaritza
Spanish State Research Agency and EDR Funds
Publisher
Springer Science and Business Media LLC
Subject
Pharmacology,General Medicine
Link
https://link.springer.com/content/pdf/10.1007/s43440-021-00253-z.pdf
Reference103 articles.
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3. Wootten D, Christopoulos A, Marti-Solano M, Babu MM, Sexton PM. Mechanisms of signalling and biased agonism in G protein-coupled receptors. Nat Rev Mol Cell Biol. 2018;19:638–53.
4. Oldham WM, Hamm HE. Heterotrimeric G protein activation by G-protein-coupled receptors. Nat Rev Mol Cell Biol. 2008;9:60–71.
5. Harrison C, Traynor JR. The [35S]GTPγS binding assay: approaches and applications in pharmacology. Life Sci. 2003;74:489–508.
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2. Ligand bias and inverse agonism on 5‐HT2A receptor‐mediated modulation of G protein activity in post‐mortem human brain;British Journal of Pharmacology;2024-04-21
3. [35S]GTPγS (Guanosine-5′-O-(γ-thio)triphosphate-[35S]) Binding Scintillation Proximity Assay Experiments in Postmortem Brain Tissue;Methods in Molecular Biology;2023
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