Viral immunoblotting: A sensitive method for detecting viral-specific oliogoclonal bands in unconcentrated cerebrospinal fluid-Reference-Cited by-同舟云学术

Viral immunoblotting: A sensitive method for detecting viral-specific oliogoclonal bands in unconcentrated cerebrospinal fluid

Published:1984-06-01 Issue:6 Volume:4 Page:505-510
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Moyle S.¹,Keir G.¹,Thompson E. J.¹

Affiliation:

1. Department of Neurochemistry, The Institute of Neurology, Queen Square, London WC1N 3BG

Abstract

A new method for detecting viral antibodies in cerebrospinal fluid is described. The technique has many advantages over previously published methods in that it is highly sensitive eliminating the need to concentrate the CSF, takes 5 h to complete, avoids the use of radionucleides, and most importantly circumvents problems associated with prozone effects which occur in immunoprecipitation reaction since the viral antigen is immobilized on nitrocellulose membranes.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/4/6/505/471009/bsr0040505.pdf

Reference13 articles.

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