The Nasal and Sinus Microbiome in Health and Disease

Author:

Wilson Michael T.,Hamilos Daniel L.

Publisher

Springer Science and Business Media LLC

Subject

Pulmonary and Respiratory Medicine,Immunology and Allergy,Immunology

Reference32 articles.

1. Lemon KP, Klepac-Ceraj V, Schiffer HK, Brodie EL, Lynch SV, Kolter R. Comparative analyses of the bacterial microbiota of the human nostril and oropharynx. mBio. 2010;1(3):e00129-10. This is a study of the bacterial microbiota of the nostril and posterior wall of the oropharynx from 7 healthy adults using a 16S rRNA gene microarray (PhyloChip) and 16S rRNA gene clone libraries showing that the PhyloChip is orders of magnitude more sensitive in its ability to identify diversity and for detecting low abundance taxa.

2. Nikolaki S, Tsiamis G. Microbial diversity in the era of omic technologies. BioMed Res Int. 2013;2013:958719. This is a excellent review of molecular tools used to study microbial communities.

3. He Z, Deng Y, Zhou J. Development of functional gene microarrays for microbial community analysis. Curr Opin Biotechnol. 2012;23(1):49–55. This is a review of functional gene arrays (FGAs), a special type of microarrays containing probes for key genes involved in microbial functional processes, and the application of FGAs to examine the human microbiome.

4. Ecker DJ, Sampath R, Massire C, Blyn LB, Hall TA, Eshoo MW, et al. Ibis T5000: a universal biosensor approach for microbiology. Nat Rev Microbiol. 2008;6(7):553–8. This study described the Ibis T5000, a platform that couples nucleic acid amplification to high-performance electrospray ionization mass spectrometry and base-composition analysis to identify pathogens in clinical and environmental samples.

5. Handelsman J, Rondon MR, Brady SF, Clardy J, Goodman RM. Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products. Chem Biol. 1998;5(10):R245-9. This paper described the use of random shotgun metagenomics, a method by which total DNA is isolated from a sample and then sequenced to profile of all genes within the community, as a means of accessing the collective genomes and the biosynthetic machinery of soil microflora.

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