Induction of Noonan syndrome-specific human-induced pluripotent stem cells under serum-, feeder-, and integration-free conditions
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Published:2020-11-02
Issue:10
Volume:56
Page:888-895
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ISSN:1071-2690
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Container-title:In Vitro Cellular & Developmental Biology - Animal
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language:en
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Short-container-title:In Vitro Cell.Dev.Biol.-Animal
Author:
Hamada Atsuko, Akagi Eri, Obayashi Fumitaka, Yamasaki Sachiko, Koizumi Koichi, Ohtaka Manami, Nishimura Ken, Nakanishi Mahito, Toratani Shigeaki, Okamoto TetsujiORCID
Abstract
AbstractNoonan syndrome is an autosomal dominant developmental disorder. Although it is relatively common, and its phenotypical variability is well documented, its pathophysiology is not fully understood. Previously, with the aim of revealing the pathogenesis of genetic disorders, we reported the induction of cleidocranial dysplasia-specific human-induced pluripotent stem cells (hiPSCs) from patient’s dental pulp cells (DPCs) under serum-free, feeder-free, and integration-free conditions. Notably, these cells showed potential for application to genetic disorder disease models. Furthermore, using similar procedures, we reported the induction of hiPSCs derived from peripheral blood mononuclear cells (PBMCs) of healthy volunteers. These methods are beneficial, because they are carried out without invasive and painful biopsies. Using those procedures, we reprogrammed DPCs and PBMCs that were derived from a patient with Noonan syndrome (NS) to establish NS-specific hiPSCs (NS-DPC-hiPSCs and NS-PBMC-hiPSCs, respectively). The induction efficiency of NS-hiPSCs was higher than that of WT-hiPSCs. We hypothesize that this was caused by high NANOG expression. Here, we describe the experimental results and findings related to NS-hiPSCs. This is the first report on the establishment of NS-hiPSCs and their disease modeling.
Funder
Japan Society for the Promotion of Science
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Developmental Biology,General Medicine
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