A protocol to effectively create single cell suspensions of adherent cells for multiparameter high-throughput flow cytometry
Author:
Publisher
Springer Science and Business Media LLC
Subject
Cell Biology,Developmental Biology,General Medicine
Link
http://link.springer.com/content/pdf/10.1007/s11626-009-9256-8.pdf
Reference22 articles.
1. Araki T.; Yamada M. Fluorescence decay measurements for determining the relative content of ethidium bromide to DNA in situ in cell nuclei. Histochemistry. 83: 299–301; 1985.
2. Brunk C. F.; Simpson L. Comparison of various ultraviolet sources for fluorescent detection of ethidium bromide-DNA complexes in polyacrylamide gels. Anal Biochem. 82: 455–62; 1977.
3. Buschmann V.; Weston K. D.; Sauer M. Spectroscopic study and evaluation of red-absorbing fluorescent dyes. Bioconjug Chem. 14: 195–204; 2003.
4. Cruickshank S. M.; Southgate J.; Selby P. J.; Trejdosiewicz L. K. Expression and cytokine regulation of immune recognition elements by normal human biliary epithelial and established liver cell lines in vitro. J. Hepatol. 29: 550–8; 1998.
5. Cui H. H.; Valdez J. G.; Steinkamp J. A.; Crissman H. A. Fluorescence lifetime-based discrimination and quantification of cellular DNA and RNA with phase-sensitive flow cytometry. Cytometry A. 52: 46–55; 2003.
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