Use of real-time PCR for determining copy number and zygosity in transgenic plants
Author:
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Agronomy and Crop Science,General Medicine
Link
http://link.springer.com/content/pdf/10.1007/s00299-004-0859-y.pdf
Reference45 articles.
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3. Bieche I, Olivi M, Champeme MH, Vidaud D, Lidereau R, Vidaud M (1998) Novel approach to quantitative polymerase chain reaction using real-time detection: application to the detection of gene amplification in breast cancer. Int J Cancer 78:661?666
4. Bubner B, Gase K, Baldwin IT (2004) Twofold differences are the detection limit for determining transgene copy numbers in plants by real-time PCR. BMC Biotechnol 4:14
5. Callaway AS, Abranches R, Scroggs J, Allen GC, Thompson WF (2002) High-throughput transgene copy number estimation by competitive PCR. Plant Mol Biol Rep 20:265?277
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