An improved MUG fluorescent assay for the determination of GUS activity within transgenic tissue of woody plants
Author:
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Agronomy and Crop Science,General Medicine
Link
http://link.springer.com/content/pdf/10.1007/s00299-002-0543-z.pdf
Reference9 articles.
1. Gallagher SR (1992) GUS protocols: using the GUS gene as a reporter of gene expression. Academic Press, San Diego
2. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387–405
3. Jefferson RA, Kavanagh TA, Bevan MW (1987) GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J 6:3901–3907
4. Klimaszewska K, Lachance D, Pelletier G, Lelu M-A, Séguin A (2001) Regeneration of transgenic Picea glauca, P. mariana, and P. abies after cocultivation of embryogenic tissue with Agrobacterium tumefaciens. In Vitro Cell Dev Biol Plant 37:748–755
5. Kosugi S, Ohashi Y, Nakajima K, Arai Y (1990) An improved assay for β-glucuronidase in transformed cells: methanol almost completely suppresses a putative endogenous β-glucuronidase activity. Plant Sci 70:133–140
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