One-step generation of zebrafish carrying a conditional knockout-knockin visible switch via CRISPR/Cas9-mediated intron targeting-Reference-Cited by-同舟云学术

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One-step generation of zebrafish carrying a conditional knockout-knockin visible switch via CRISPR/Cas9-mediated intron targeting

Published:2019-12-20 Issue:1 Volume:63 Page:59-67
ISSN:1674-7305
Container-title:Science China Life Sciences
language:en
Short-container-title:Sci. China Life Sci.

Author:

Li Jia,Li Hong-Yu,Gu Shan-Ye,Zi Hua-Xing,Jiang Lai,Du Jiu-Lin

Publisher

Springer Science and Business Media LLC

Subject

General Agricultural and Biological Sciences,General Environmental Science,General Biochemistry, Genetics and Molecular Biology

Link

http://link.springer.com/content/pdf/10.1007/s11427-019-1607-9.pdf

Reference24 articles.

1. Bertrand, J.Y., Chi, N.C., Santoso, B., Teng, S., Stainier, D.Y.R., and Traver, D. (2010). Haematopoietic stem cells derive directly from aortic endothelium during development. Nature 464, 108–111.

2. Chang, N., Sun, C., Gao, L., Zhu, D., Xu, X., Zhu, X., Xiong, J.W., and Xi, J.J. (2013). Genome editing with RNA-guided Cas9 nuclease in zebrafish embryos. Cell Res 23, 465–472.

3. Dai, J., Cui, X., Zhu, Z., and Hu, W. (2010). Non-homologous end joining plays a key role in transgene concatemer formation in transgenic zebrafish embryos. Int J Biol Sci 6, 756–768.

4. Gibb, N., Lazic, S., Yuan, X., Deshwar, A.R., Leslie, M., Wilson, M.D., and Scott, I.C. (2018). Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development. Development 145, dev167510.

5. Hoshijima, K., Jurynec, M.J., and Grunwald, D.J. (2016). Precise editing of the zebrafish genome made simple and efficient. Dev Cell 36, 654–667.

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