A Real-Time Quantitative PCR Protocol for the Quantification of Plasmid Copy Number in Lactococcus lactis
Author:
Publisher
Springer US
Link
https://link.springer.com/content/pdf/10.1007/978-1-0716-3358-8_12
Reference3 articles.
1. Carapuça E, Azzoni AR, Prazeres DM, Monteiro GA, Mergulhao FJ (2007) Time-course determination of plasmid content in eukaryotic and prokaryotic cells using real-time PCR. Mol Biotechnol 37(2):120–126
2. Skulj M, Okrslar V, Jalen S, Jevsevar S, Slanc P, Strukelj B, Menart V (2008) Improved determination of plasmid copy number using quantitative real-time PCR for monitoring fermentation processes. Microb Cell Factories 7:6. https://doi.org/10.1186/1475-2859-7-6
3. Duarte SOD, Martins MC, Andrade SM, Prazeres DMF, Monteiro GA (2019) Plasmid copy number of pTRKH3 in Lactococcus lactis is increased by modification of the repDE ribosome-binding site. Biotechnol J 14(8):e1800587. https://doi.org/10.1002/biot.201800587. Epub 2019 May 20. PMID: 31009171
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