Floxing by Electroporating Single-Cell Embryos with Two CRISPR RNPs and Two ssODNs
Author:
Publisher
Springer US
Link
https://link.springer.com/content/pdf/10.1007/978-1-0716-2990-1_9
Reference20 articles.
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3. Yang H, Wang H, Shivalila CS, Cheng AW, Shi L, Jaenisch R (2013) One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering. Cell 154:1370–1379
4. Bishop KA, Harrington A, Kouranova E et al (2016) CRISPR/Cas9-mediated insertion of loxP sites in the mouse dock7 gene provides an effective alternative to use of targeted embryonic stem cells. G3 6:2051–2061
5. Pritchard CEJ, Kroese LJ, Huijbers IJ (2017) Direct generation of conditional alleles using CRISPR/Cas9 in mouse zygotes. Methods Mol Biol 1642:21–35
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