AIRR Community Guide to Planning and Performing AIRR-Seq Experiments-Reference-Cited by-同舟云学术

AIRR Community Guide to Planning and Performing AIRR-Seq Experiments

Published:2022 Issue: Volume: Page:261-278
ISSN:1064-3745
Container-title:Methods in Molecular Biology
language:
Short-container-title:

Author:

Eugster Anne^ORCID,Bostick Magnolia L.^ORCID,Gupta Nidhi^ORCID,Mariotti-Ferrandiz Encarnita^ORCID,Kraus Gloria,Meng Wenzhao,Soto Cinque^ORCID,Trück Johannes^ORCID,Stervbo Ulrik^ORCID,Luning Prak Eline T.^ORCID,

Abstract

AbstractThe development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.

Publisher

Springer US

Link

https://link.springer.com/content/pdf/10.1007/978-1-0716-2115-8_15

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