Analysis of DNA Double-Stranded Breaks Using the Comet Assay in Planarians-Reference-Cited by-同舟云学术

Analysis of DNA Double-Stranded Breaks Using the Comet Assay in Planarians

Published:2022 Issue: Volume: Page:479-491
ISSN:1064-3745
Container-title:Methods in Molecular Biology
language:
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Author:

Barghouth Paul G.,Rojas Salvador,O’Dell Lacey R.,Betancourt Andrew M.,Oviedo Néstor J.

Abstract

AbstractComet assay provides the opportunity to detect and characterize DNA strand breaks. Cellular lysing followed by embedding in agarose slide is used to visualize under an electrical current migration patterns corresponding to DNA fragments of different sizes. Here we describe the process of detecting and characterizing DNA damage by Comet assay on planarians, which is a model organism commonly used to understand the process of whole-body regeneration, stem cell regulation, and adult tissue maintenance.

Publisher

Springer US

Link

https://link.springer.com/content/pdf/10.1007/978-1-0716-2172-1_25

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