Quantitative Real-Time PCR Method to Evaluate Gene Expression in Zebrafish Embryos
Author:
Beler MerihORCID,
Cansız DeryaORCID,
Ünal İsmailORCID,
Emekli-Alturfan EbruORCID
Reference7 articles.
1. Schmittgen T, Livak K (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3:1101–1108. https://doi.org/10.1038/nprot.2008.73
2. Vanhauwaert S, Lefever S, Coucke P, Speleman F, De Paepe A, Vandesompele J, Willaert A (2016) RT-qPCR gene expression analysis in zebrafish: preanalytical precautions and use of expressed repetitive elements for normalization. Methods Cell Biol 135:329–342. https://doi.org/10.1016/bs.mcb.2016.02.002. Epub 2016 Mar 4. PMID: 27443934
3. Zhu H, Zhang H, Xu Y, Laššáková S, Korabečná M, Neužil P (2020) PCR past, present and future. Biotechniques 69(4):317–325. https://doi.org/10.2144/btn-2020-0057. Epub 2020 Aug 20. PMID: 32815744; PMCID: PMC7439763
4. Haddad F, Baldwin KM (2010) Reverse transcription of the ribonucleic acid: the first step in RT-PCR assay. Methods Mol Biol 630:261–270. https://doi.org/10.1007/978-1-60761-629-0_17. PMID: 20301003
5. Üstündağ FD, Ünal İ, Üstündağ ÜV et al (2022) 3-Pyridinylboronic acid ameliorates rotenone-induced oxidative stress through Nrf2 target genes in zebrafish embryos. Neurochem Res 47:1553–1564. https://doi.org/10.1007/s11064-022-03548-6