Profiling Transcriptional Heterogeneity with Seq-Well S3: A Low-Cost, Portable, High-Fidelity Platform for Massively Parallel Single-Cell RNA-Seq

Author:

Drake Riley S.,Villanueva Martin Arreola,Vilme Mike,Russo Daniela D.,Navia Andrew,Love J. Christopher,Shalek Alex K.

Publisher

Springer US

Reference29 articles.

1. Gierahn TM, Wadsworth MH 2nd, Hughes TK, Bryson BD, Butler A, Satija R, Fortune S, Love JC, Shalek AK (2017) Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput. Nat Methods 14(4):395–398. https://doi.org/10.1038/nmeth.4179

2. Hughes TK, Wadsworth MH 2nd, Gierahn TM, Do T, Weiss D, Andrade PR, Ma F, de Andrade Silva BJ, Shao S, Tsoi LC, Ordovas-Montanes J, Gudjonsson JE, Modlin RL, Love JC, Shalek AK (2020) Second-Strand synthesis-based massively parallel scRNA-Seq reveals cellular states and molecular features of human inflammatory skin pathologies. Immunity 53(4):878–894.e7. https://doi.org/10.1016/j.immuni.2020.09.015

3. Klein AM, Mazutis L, Akartuna I, Tallapragada N, Veres A, Li V, Peshkin L, Weitz DA, Kirschner MW (2015) Droplet barcoding for single-cell transcriptomics applied to embryonic stem cells. Cell 161(5):1187–1201. https://doi.org/10.1016/j.cell.2015.04.044

4. Macosko EZ, Basu A, Satija R, Nemesh J, Shekhar K, Goldman M, Tirosh I, Bialas AR, Kamitaki N, Martersteck EM, Trombetta JJ, Weitz DA, Sanes JR, Shalek AK, Regev A, McCarroll SA (2015) Highly parallel genome-wide expression profiling of individual cells using Nanoliter droplets. Cell 161(5):1202–1214. https://doi.org/10.1016/j.cell.2015.05.002

5. Montoro DT, Haber AL, Biton M, Vinarsky V, Lin B, Birket SE, Yuan F, Chen S, Leung HM, Villoria J, Rogel N, Burgin G, Tsankov AM, Waghray A, Slyper M, Waldman J, Nguyen L, Dionne D, Rozenblatt-Rosen O, Tata PR, Mou H, Shivaraju M, Bihler H, Mense M, Tearney GJ, Rowe SM, Engelhardt JF, Regev A, Rajagopal J (2018) A revised airway epithelial hierarchy includes CFTR-expressing ionocytes. Nature 560(7718):319–324. https://doi.org/10.1038/s41586-018-0393-7

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