Author:
Kuo Yi-Tzu,Kurian Jacob Gigi,Schubert Veit,Fuchs Jörg,Melzer Michael,Muraleedharan Ananthu,Maruthachalam Ravi,Houben Andreas
Abstract
AbstractHolocentric species are characterized by the presence of centromeres throughout the length of the chromosomes. We confirmed the holocentricity of the dioecious, small chromosome-size species Myristica fragrans based on the chromosome-wide distribution of the centromere-specific protein KNL1, α-tubulin fibers, and the cell cycle-dependent histone H3 serine 28 phosphorylation (H3S28ph) mark. Each holocentromere is likely composed of, on average, ten centromere units, but none of the identified and in situ hybridized high-copy satellite repeats is centromere-specific. No sex-specific major repeats are present in the high-copy repeat composition of male or female plants, or a significant difference in genome size was detected. Therefore, it is unlikely that M. fragrans possesses heteromorphic sex chromosomes.
Funder
DFG
DAAD
DAAD-WISE Scholarship
Dupont Young Professor Grant
IISER TVM intramural support
Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK)
Publisher
Springer Science and Business Media LLC