A Novel Method to Improve the Physical Property and Biocompatibility of Decellularized Heart Valve Scaffold with Sericin and Polydopamine
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Published:2022-06-13
Issue:4
Volume:19
Page:1109-1123
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ISSN:1672-6529
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Container-title:Journal of Bionic Engineering
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language:en
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Short-container-title:J Bionic Eng
Author:
Bai Peng, Kong Gangcheng, Qiao Weihua, Song Yu, Wang Yixuan, Shi Jiawei, Zhang Nan, Liu Chungeng, Chu Chong, Xiong Tixiusi, Zhou Ying, Lu Cuifen, Wang Lin, Dong NianguoORCID
Abstract
AbstractCardiac valve replacement is an effective method to treat valvular heart disease. Artificial valves used routinely in clinic still have defects. In our study, we explored a novel method to modify the performance of Decellularized Heart Valve (DHV) scaffold. The decellularized porcine aortic valve was prepared using sequential hydrophile and lipophile solubilization method. The sericin was extracted from silk fibroin-deficient silkworm cocoon by lithium bromide method. First, DHV was immersed in sericin solution to produce the sericin–DHV composite scaffold. Then, we modified the DHV by making a Polydopamine (PDA) coating on the DHV first and then binding the sericin. The physical properties and biological compatibility of our composite scaffold were assessed in vitro and in vivo. Sericin were successfully prepared, combined to DHV and improved its biocompatibility. PDA coating further promoted the combination of sericin on DHV and improved the physical properties of scaffolds. The decay rate of our modified valve scaffold was decreased in vivo and it showed good compatibility with blood. In conclusion, our modification improved the physical properties and biocompatibility of the valve scaffold. The combination of PDA and sericin promoted the recellularization of decellularized valves, showing great potential to be a novel artificial valve.
Funder
National Key Research and Development Program of China Stem Cell and Translational Research National Natural Science Foundation of China
Publisher
Springer Science and Business Media LLC
Subject
Bioengineering,Biophysics,Biotechnology
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