Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter
Author:
Publisher
Humana Press
Link
http://link.springer.com/content/pdf/10.1007/978-1-62703-293-3_17
Reference16 articles.
1. Graslund S, Nordlund P, Weigelt J et al (2008) Protein production and purification. Nat Methods 5:135–146
2. Peleg Y, Unger T (2012) Resolving bottlenecks for recombinant protein expression in E. coli. Methods Mol Biol 800:173–186
3. Dale GE, Broger C, Langen H et al (1994) Improving protein solubility through rationally designed amino acid replacements: solubilization of the trimethoprim-resistant type S1 dihydrofolate reductase. Protein Eng 7:933–939
4. Sun L, Petrounia IP, Yagasaki M et al (2001) Expression and stabilization of galactose oxidase in Escherichia coli by directed evolution. Protein Eng 14:699–704
5. Leong SR, Chang JC, Ong R et al (2003) Optimized expression and specific activity of IL-12 by directed molecular evolution. Proc Natl Acad Sci U S A 100:1163–1168
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