Affinity determination of biomolecules: a kinetic model for the analysis of pre-equilibrium titration curves
Author:
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Biophysics
Link
http://link.springer.com/article/10.1007/s00249-018-1318-y/fulltext.html
Reference9 articles.
1. Ducata DD, Jaehrling J, Hänel C, Satzger M, Wolber M, Ostendorp R, Pabst S, Brocks B (2015) Solution equilibrium titration for high-throughput affinity estimation of unpurified antibodies and antibody fragments. J Biomol Screen 20:1256–1267
2. Estep P, Reid F, Nauman C, Liu Y, Sun T, Sun J, Xu Y (2013) High throughput solution-based measurement of antibody-antigen affinity and epitope binning. mAbs 5:270–278
3. Haenel C, Satzger M, Ducata DD, Ostendorp R, Brocks B (2005) Characterization of high-affinity antibodies by electrochemiluminescence-based equilibrium titration. Anal Biochem 339:182–184
4. Hardy F, Djavadi-Ohaniance L, Goldberg ME (1997) Measurement of antibody/antigen association rate constants in solution by a method based on the enzyme-linked immunosorbent assay. J Immunol Methods 200:155–159
5. Karlsson R (1994) Real-time competitive kinetic analysis of interactions between low-molecular-weight ligands in solution and surface-immobilized receptors. Anal Biochem 221:142–151
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