Optimization of cell culture conditions for G-CSF (granulocyte-colony stimulating factor) production by genetically engineered namalwa KJM-1 cells

Author:

Hosoi Shinji,Murosumi Kazunari,Sasaki Katsutoshi,Satoh Mitsuo,Miyaji Hiromasa,Hasegawa Mamoru,Itoh Seiga,Tamaoki Tatsuya,Sato Seiji

Publisher

Springer Science and Business Media LLC

Subject

Cell Biology,Clinical Biochemistry,Biomedical Engineering,Bioengineering,Biotechnology

Reference19 articles.

1. Dorai H and Moore GP (1987) The effect of dihydrofolate reductase-mediated gene amplification on the expression of transfected immunoglobulin genes. J. Immunol. 139: 4232?4241.

2. Hosoi S, Mioh H, Anzai C, Sato S and Fujiyoshi N (1988) Establishment of Namalwa cell lines which grow continuously in glutamine-free medium. Cytotechnology 1: 151?158.

3. Kaufman RJ and Sharp PA (1982) Amplification and expression of sequences cotransfected with a modular dihydrofolate reductase complementary DNA gene. J. Mol. Biol. 159: 601?621.

4. Kaufman RJ, Wasley LC, Spiliotes AJ, Gossels SD, Latt SA, Larsen GR and Kay RM (1985) Coamplification and coexpression of human tissue-type plasminogen activator and murine dihydrofolate reductase sequences in Chinese hamster ovary cells. Mol. Cell. Biol. 5: 1750?1759.

5. Komatsu Y, Matsumoto T, Kuga T, Nishi T, Sekine S, Saito A, Okabe M, Morimoto M, Itoh S, Okabe T and Takaku F (1987) Cloning of granulocyte colony-stimulating factor cDNA from human macrophages and its expression in Escherichia coli. Jpn. J. Cancer Res. 78: 1179?1181.

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