Chromatin structure of genes during the cell cycle

Author:

Jing-de Zhu123,Paul John1

Affiliation:

1. The Beatson Institute for Cancer Research, Garscube Estate, Switchback Road, Bearsden, Glasgow G61 1BD, Scotland

2. Washington University in St. Louis, Department of Biology, Campus Box 1137, St. Louis, MO 63130, USA

3. Shanghai Institute for Cell Biology, 320 Yue Yang Road, Shanghai, The People's Republic of China

Abstract

K562 ceils were separated by centrifugal elutriation into 5 fractions each of which had a unique distribution of ceils at different phases of the cell cycle. A study of DNAseI sensitivity of the e- and γ-globin genes, which are expressed in these cells, and the λ light chain immunoglobulin gene, which is not expressed, in nuclei from each of the 5 fractions was undertaken. There was no detectable difference in the arrangement of DNAseI-hypersensitive sites (DHS) in chromatin of each of the three genes in the different fractions.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

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