Multiwell cap assay: A simple objective method for the assessment of leukocyte locomotion in vitro-Reference-Cited by-同舟云学术

Multiwell cap assay: A simple objective method for the assessment of leukocyte locomotion in vitro

Published:1986-12-01 Issue:12 Volume:6 Page:1041-1047
ISSN:0144-8463
Container-title:Bioscience Reports
language:en
Short-container-title:

Author:

Evans Anne M.¹,David Chambers J.¹,Hobbs John R.¹

Affiliation:

1. Department of Chemical Immunology, Charing Cross and Westminster Medical School, 17 Page Street, London SW1 P 2AP, UK

Abstract

A simple method for evaluating leukocyte locomotion in vitro has been developed and validated for several chemoattractants. The multiwell cap assay (MWCA) comprises chambers constructed from readily available disposable plastics and is quickly assembled, permitting large experimental protocols. Leukocytes which have migrated through a micropore filter are recovered and counted electronically yielding a precise, objective result. Coefficients of variation are approximately 6%.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

Link

https://portlandpress.com/bioscirep/article-pdf/6/12/1041/471491/bsr0061041.pdf

Reference22 articles.

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