Molecular forms of the cholinesterases inside and outside muscle endplates

Author:

Jedrzejczyk Jolanta12,Silman Israel13,Lyles Joan M.1,Barnard Eric A.1

Affiliation:

1. Department of Biochemistry, Imperial College, London SW7 2AZ, U.K.

2. Department of Histology, Medical Academy, Warsaw, Poland

3. Department of Neurobiology, Weizmann Institute of Science, Renovot, Israel

Abstract

Individual endplates were micro-dissected from chicken fast-twitch muscle, and the molecular forms of acetylcholinesterase and of pseudocholinesterase therein, identified by their sedimentation coefficients, were analysed directly. The forms actually present at the endplate, and those that are non-synaptic9 were established. This analysis was also extended to muscle of the chicken with inherited muscular dystrophy, showing altered distributions of these forms.

Publisher

Portland Press Ltd.

Subject

Cell Biology,Molecular Biology,Biochemistry,Biophysics

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