Abstract
AbstractTemperature is an important environmental factor for phytoplankton. Phytoplankton growth, metabolism, biodiversity, productivity, and distribution are influenced by temperature-driven nutrient stratification and mixing, as well as species’ optimal growth temperatures. There have been a number of studies focused on physiological and biochemical mechanisms of environmental–biological interactions in diatoms, yet the underlying transcriptional regulators remain limited. Here, we performed an RNA-seq-based gene expression analysis to explore the Skeletonema marinoi (isolated from Jiaozhou Bay of Qingdao, 36.13°N, 120.16°E on July 5th, 2013) cellular responses induced by low temperature (12 °C). Digital gene expression profiling of S. marinoi generated 20,319 unigenes, of which 573 differentially expressed genes appeared in the low-temperature treatment group. According to GO and KEGG enrichment analysis, different genes were involved in ten metabolic and biosynthesis pathways: ribosome, lipid, porphyrin, and chlorophyll metabolism showed strong transcriptional cold tolerances. The regulation of genes related to translation processes (e.g., pentatricopeptide repeat), fatty acid metabolism (e.g., acyl-CoA synthetase), and photosynthesis (e.g., porphyrin enzymes) provides new molecular-level insight into cold stress responses in eukaryotic marine phytoplankton. Our study suggests that this Skeletonema species could be a potential candidate for understanding the fate of thermo-sensitive diatom communities and oceanic ecosystems facing climate change.
Funder
National Key Research and Development Program of China
the Scientific and Technological Innovation Project of the Qingdao National Laboratory for Marine Science and Technology
Natural Science Foundation of Shandong Province
Publisher
Springer Science and Business Media LLC
Reference55 articles.
1. Anders S, Huber W (2010) Differential expression analysis for sequence count data. Genome Biol 11:R106
2. Behrenfeld MJ, Omalley RT, Siegel DA, McClain CR, Sarmiento JL, Feldman GC, Milligan AJ, Falkowski PG, Letelier RM, Boss ES (2006) Climate-driven trends in contemporary ocean productivity. Nature 444:752–755
3. Bierkens J, Maes J, Plaetse FV (1998) Dose-dependent induction of heat shock protein 70 synthesis in Raphidocelis subcapitata following exposure to different classes of environmental pollutants. Environ Pollut 10:191–197
4. Boyce DG, Lewis RM, Worm B (2010) Global phytoplankton decline over the past century. Nature 466:591–596
5. Cvetkovska M, Szyszka-Mroz B, Possmayer M, Pittock P, Lajoie G, Smith DR, Hüner NPA (2018) Characterization of photosynthetic ferredoxin from the antarctic alga Chlamydomonas sp. UWO241 reveals novel features of cold adaptation. New Phytol 219:588–604