Author:
Jiang Liyuan,Li Chengjun,Li Miao,Yin Xianzhen,Wu Tianding,Duan Chunyue,Cao Yong,Lu Hongbin,Hu Jianzhong
Abstract
AbstractEffective methods for visualizing neurovascular morphology are essential for understanding the normal spinal cord and the morphological alterations associated with diseases. However, ideal techniques for simultaneously imaging neurovascular structure in a broad region of a specimen are still lacking. In this study, we combined Golgi staining with angiography and synchrotron radiation micro-computed tomography (SRμCT) to visualize the 3D neurovascular network in the mouse spinal cord. Using our method, the 3D neurons, nerve fibers, and vasculature in a broad region could be visualized in the same image at cellular resolution without destructive sectioning. Besides, we found that the 3D morphology of neurons, nerve fiber tracts, and vasculature visualized by SRμCT were highly consistent with that visualized using the histological method. Moreover, the 3D neurovascular structure could be quantitatively evaluated by the combined methodology. The method shown here will be useful in fundamental neuroscience studies.
Publisher
Springer Science and Business Media LLC
Subject
General Medicine,Physiology,General Neuroscience
Cited by
11 articles.
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