Development of real-time PCR assay using TaqMan probe for detection and quantification of Rosellinia necatrix in plant and soil
Author:
Publisher
Springer Science and Business Media LLC
Subject
Plant Science,Agronomy and Crop Science
Link
http://link.springer.com/content/pdf/10.1007/s10327-012-0366-x.pdf
Reference16 articles.
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2. Cullen DW, Hirsch PR (1998) Simple and rapid method for direct extraction of microbial DNA from soil for PCR. Soil Biol Biochem 30:983–993
3. Eguchi N, Kondo K, Yamagishi N (2009) Bait twig method for soil detection of Rosellinia necatrix, causal agent of white root rot of Japanese pear and apple, at an early stage of tree infection. J Gen Plant Pathol 75:325–330
4. Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 61:1323–1330
5. Heid CA, Stevens J, Livak KJ, Williams PM (1996) Real-time quantitative PCR. Genome Res 6:986–994
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