Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers-Reference-Cited by-同舟云学术

Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers

Published:2012-09-14 Issue:1 Volume:90 Page:43-50
ISSN:1099-209X
Container-title:American Journal of Potato Research
language:en
Short-container-title:Am. J. Potato Res.

Author:

Singh Mathuresh,Singh Rudra P.,Fageria M. S.,Nie X.,Coffin Robert,Hawkins G.

Publisher

Springer Science and Business Media LLC

Subject

Agronomy and Crop Science,Plant Science

Link

http://link.springer.com/content/pdf/10.1007/s12230-012-9274-z.pdf

Reference28 articles.

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2. Bustin, S.A. 2000. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. Journal of Molecular Endocrinology 25: 169–193.

3. Ellis, P., R. Stace-Smith, G. Bowler, and D.J. Mackenzie. 1996. Production of monoclonal antibodies for detection and identification of strains of potato virus Y. Canadian Journal of Plant Pathology 18: 64–70.

4. Fageria, M.S., M. Singh, U. Nanayakkara, D. Wattie, Y. Pelletier, and X. Nie. 2012. Monitoring current season spread of Potato virus Y in seed potato fields. American Journal of Potato Research 89: 30–52 (Abstract).

5. Gray, S., S. De Boer, J. Lorenzen, A. Karasev, J. Whitworth, P. Nolte, R. Singh, A. Boucher, and H. Xu. 2010. Potato virus Y: an evolving concern for potato crops in the United States and Canada. Plant Disease 94: 1384–1397.

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