Affiliation:
1. Robert Koch-Institut des Bundesgesundheitsamtes, Nordufer 20, D-1000 Berlin 65, Germany
Abstract
Scrapie hamster brains contain at least 5–10 μg of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differentia] centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.
Subject
Cell Biology,Molecular Biology,Biochemistry,Biophysics
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