SYNERGISTIC ANTIMICROBIAL ACTIVITY BY COMBINING TERBINAFINE WITH BENZOYL PEROXIDE AGAINST САNDIDA ALBICANS AND STAPHYLOCOCCUS AUREUS

Abstract

The aim. To investigate the ability of microorganisms’ C. albicans and S. aureus to form biofilms. To evaluate the sensitivity of biofilms to combination of terbinafine and benzoyl peroxide on this association. Material and methods. The ability to form biofilms of microorganisms and the antimicrobial effect of the studied drugs was carried out on polystyrene plates for enzyme-linked immunosorbent assay. The optical density (OD) of biofilms was measured at a wavelength of 545 nm on a biochemical analyzer. The viability of microorganisms was determined by counting the number of colony-forming units (CFU) in 1 ml of the culture medium with experimental strains. Results. The average optical density of biofilms was (1.0892±0.006) units. The OD for clinical isolates was (0.0776±0.004) units. It has been proven that combination of the antimycotic substance − terbinafine and the antiseptic − benzoyl peroxide had a high activity in relation to the association of C. albicans and S. aureus with concentration of 1.3 μg/ml. Conclusion. The study showed the ability to form biofilms in clinical strains of microorganisms is more pronounced than the reference strains. The highest rate of biofilm formation was found in the association of microorganisms’ C. albicans and S. aureus. The combination of medicines also effectively operated with planktonic forms of bacteria, but also on microorganisms mobilized in biofilms. A decrease of optical density and 2-fold decrease of CFU demonstrated this.