PHENOTYPIC IDENTIFICATION OF EXTENDED SPECTRUM BETA LACTAMASE AND METALLO BETA LACTAMASE PRODUCTION AMONG ISOLATES OF ENTEROBACTERIACEAE IN A TERTIARY CARE HOSPITAL, UDAIPUR, RAJASTHAN

Abstract

Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for more concern among Enterobacteriaceae. The prevalence of extended spectrum -lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current situation of -lactam therapy leading to treatment failure. 100 Enterobacteriaceae isolated from various clinical samples were included in the study. ESBL was detected by phenotypic conrmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid. MBLdetection was done by Imipenem EDTA combined disc diffusion test. Out of 100 Enterobacteriaceae isolates, 47(47%) were ESBL producers and 29 (29%) were MBLproducers. None of the isolates showed the coexistence of ESBLand MBLin the same isolate. ESBLand MBLproduction was observed in E.coli, Klebsiella spp., Acinetobacter spp. and Citrobacter spp. isolated from various clinical samples. The study underlines problem of ESBL and MBL mediated resistance, which has created a therapeutic challenge for the clinicians and microbiologists. Simple disk method can be routinely employed to detect these common resistance mechanisms which will reduce the mortality and also spread of such resistant strains.