COMPARISON OF HUMANPAPILLOMAVIRUS MESSANGER(E6)RNA EXPRESSION AND DNA AS BIOMARKER IN SCREENING OF VIAVILI POSITIVE CASES IN TERTIARY CARE

Abstract

Cervical cancer is second most frequent cancer of women in the world. HPV infection is now a well established cause of cervical cancer . Currently used screening programmes are detecting cases in which only 15-20 percent of women found to have an abnormality signicant enough to need treatment, other 80-85 percent were probably false alarms. The oncogenic process in cervical cancer is initiated and mediated by upregulation of E6/E7 mRNA proteins and their over expression. So HPV E6 mRNA detection as a triage test could actually detect a neoplastic transformation and may help to reduce the false alarms. To compare the expression of HPV E6 mRN AIMS AND OBJECTIVES : A verses the HPV DNA as a biomarker in the screening of cervical cancer .Biopsy is used as gold standard for screening. This a prospective MATERIALSAND METHODS: study conducted in Institute of Social Obstetrics and Govt. Kasturba Gandhi hospital, Madras medical college, 41 cases positive for VIA/VILI in colposcopy were selected and subjected to conventional cytology and cervical biopsy ,part of biopsy was collected in RNA later solution and were typed for HPV DNA 16& 18,HPV E6mRNA Expression.the results of above test are compared with histopathology as gold standard. RESULTS: The specicity of E6 mRNA was 100% for both high grade and low grade lesions.But the sensitivity was less than HPV DNA and cytology for both low and high grade lesions.With increasing severity of lesion expression of E6 mRNA was found to increase. Low positivity CONCLUSION: rate and the high specicity makes E6 mRNA better biomarker in a screening of cervical cancer.