EXPERIMENTAL EVALUATION OF ANXIOLYTIC ACTIVITY OF ALCOHOLIC EXTRACT OF CITRUS LIMON LEAVES ON RATS.

Abstract

INTRODUCTION : Anxiety as a disorder is increasing worldwide due to complexity of daily life in modern society. In India it is reported to be highest among all psychiatry disorders, thus highlighting the importance of treating anxiety disorders. Benzodiazepines are widely prescribed drugs for treatment but their clinical uses are limited due to side effects. Citrus Limon is a highly reputed plant and has been widely employed in complementary and alternative medicine but no signicant work has been carried out on the anxiolytic effects of this plant extract.The present study was designed to experimentally evaluate the anxiolytic effects of ethanolic extract of leaves of Citrus Limon on wistar rats using three different doses orally i.e 50,100,200 mg/kg on open eld test.. MATERIAL& METHODS: Atotal of 30 wistar rats of either sex were divided into 5 groups with 6 rats each. Group I(control): received distilled water. Group II(standard):Diazepam 2mg/kg is given. Group III -50mg/kg of extract is given. Group IV-100mg/kg is given. Group V- 200mg/kg is given. Data analyzed by one way ANOVA and Post hoc comparisions were performed by Tukey Kramer multiple comparision test.P< 0.05 was considered signicant. RESULTS : Ethanolic extract of Citrus Limon in dose of 100mg/kg showed effective anxiolytic activity compared to 50,200 mg/kg dose and effect is compared to Diazepam. CONCLUSION:The present study suggest that Citrus Limonmay be developed as a potential anti anxiety agent.