EVALUATION OF ANTI-INFLAMMATORY ACTIVITIES OF ALCOHOLIC EXTRACT OF CUSCUTA REFLEXA ROXB. STEM ON EXPERIMENTAL ANIMALS

Abstract

Aim of the study: To evaluate the anti-Inammatory activity of alcoholic extract of Cuscuta reexa stem on experimental animals Stems of Cuscuta reexa were collected, air dried and powdered.Materials and Methods: Methanolic extract was obtained by percolating the dried powder with 99.8% methanol. Alcoholic extracts of Cuscuta reexa stem (100mg/kg) was used as test drug. Anti-inammatory activity was on acute inammation was evaluated by carrageenan induced rat paw oedema method and chronic inammation was studied by Freund's complete adjuvant-induced arthritis method. For each of the studies albino rats of either sex weighing 100-200gms were divided into 3 groups with 5 animals in each group. Group A received normal saline 10 ml/kg. Test groups B, received alcoholic extracts of Cuscuta reexa stem (100mg/kg), Standard group C received Aspirin (100mg/kg). Acute oral toxicity test of the extract was performed as per OECD 425 (OECD Guidelines, 2001). The results were analysed by ANOVA followed by Dunnett'sResults: multiple comparison test. Alcoholic extracts of Cuscuta reexa stem (100mg/kg) showed signicant (p<0.01) anti-inammatory activity when compared to the control in carrageenan induced acute inammation. AECR was also effective in chronic arthritis model. TheConclusion: present study indicates that alcoholic extracts of Cuscuta reexa stem (100mg/kg) showed signicant (p<0.01) anti-inammatory activity against acute inammation as well as chronic inammation.