INFLUENCE OF CHITOSAN SOLUTION ON THE VIABILITY OF ARBUSCULAR MYCORRHIZAL SPORES-Reference-Cited by-同舟云学术

INFLUENCE OF CHITOSAN SOLUTION ON THE VIABILITY OF ARBUSCULAR MYCORRHIZAL SPORES

Published:2021-07-01 Issue: Volume: Page:7-9
ISSN:
Container-title:INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH
language:en
Short-container-title:ijsr

Author:

Singh Nalini¹,Chahar Sunita²

Affiliation:

1. Research Scholar NES Ratnam College Of Arts, Science & Commerce, Bhandup(West), Mumbai -78.

2. Head, Department of Botany NES Ratnam College Of Arts, Science & Commerce, Bhandup(West), Mumbai -78

Abstract

The study was aimed to fortify the Arbuscular Mycorrhizal Fungal (AMF) spores, widely used in Bio-fertilizers with chitosan and check the inuence of chitosan on the viability of AMF spores. Chitosan was prepared from shrimp shells using chemical method involving demineralization, deproteinization and deacetylation.AMF spores were kept in three different concentrations of chitosan (0.1%, 0.5 %and 1%) which was prepared in 0.1% acetic acid and 0.1% ascorbic acid. Spore viability was checked by the MTT 3-(4, 5 th th th dimethylthiazol-yl-2, 5-diphenyl-2H-tetrazolium bromide) after a day, 10 day, 20 day and 30 day. Highest number of viable spores was observed in chitosan dissolved in 0.1% ascorbic acid as compared to chitosan in acetic acid.

Publisher

World Wide Journals

Reference19 articles.

1. Aizi, D. and Cheba, B.A. (2015). Influence of chitinous waste on soil bacterial community: Biofertilizer effect and antifungal activity. Procedia Technology. 965-971.

2. An, Z.Q. and Hendrix, J.W.(1988). Determining viability of Endogonaceous spores with a vital stain. Mycologia. 80(2): 259-261.

3. Bansal, M.,Jagpal, R. and Mukerji, K. G.(1991).Viability of VAM fungal spores. RecentAdvancesinAppliedSciences.6: 65–68.

4. Baum, C., El-Tohamy,W. and Gruda, N. (2015). Increasing the productivity and product quality of vegetable crops usingArbuscularmycorrhizal fungi: a review. Sci. Hortic. 187: 131–141.

5. Berruti, A., Lumini, E., Balestrini, R. and Bianciotto, V. (2016). ArbuscularMycorrhizal Fungi as Natural Biofertilizers: Let's Benefit from Past Successes. Frontiers in microbiology.6: 1559.