Affiliation:
1. Research scholar, Department of Bio-Technology, Bharath Institute of Higher Education and Research.
2. Professor, Department of Agricultural Bio-Technology, Bharath Institute of Higher Education and Research.
Abstract
The main objective of this study is to investigate the cytotoxic potential of four puried compounds (compounds 1,2,3 and
4) from medicinal plant Chrysopogon zizanioides L were investigated. Successive solvent extraction of Chrysopogon
zizanioides in chloroform was done. The extract were tested against the normal cell lines (Vero) human colorectal cancer cell lines (HCT-116)
using the thiazolyl blue test (MTT) assay. The chloroform fractions of Chrysopogon zizanioides had shown the signicant results against Vero and
HCT-116 tumor cell lines. The IC50concentration of the selected puried compounds C1, C2, C3, and C4 against HCT 116 were 79.06 µg/ml,
84.13 µg/ml, 94.36 µg/ml and 68.47µg/ml, respectively. Whereas the IC50concentration of the selected puried compounds C1, C2, C3, and C4
against Vero were 130.30µg/ml, 157.36µg/ml, 160.48µg/ml and 255.28µg/ml, respectively. Among all the tested compounds, compound four can
be considered as potential sources of anti-cancer compounds. Additional studies are necessary for more extensive biological evaluations.