STUDY OF EFFECTIVENESS OF SOLUBILITY TEST FOR SICKLE CELL DISEASE IN FEMALES IN REPRODUCTIVE AGE GROUP AS SCREENING TOOL AND HEMATOLOGICAL CHANGES DURING ANTENATAL VISITS – INSTITUTIONAL ANALYSIS.

Abstract

Sickle cells Disease is a result from inheritance of sickle cell gene that codes for Beta- globin chain. This change leads to single base A →T in the sixth codon of Beta globin gene so that there is substitution of thymine for adenine, this in turn causes substitution of valine for glutamic acid at position 6 of β polypeptide chain. The purpose of the Retrospective study is to identify Context: unknown identities in Tribal Zone having Sickle cell Disease as well to trace such females in Reproductive age groups. Utility of Aims: Solubility test in early detection and identifying the cases with Sickle disease and reliability of the Methodology. Study Setting And Design: conducted on females with different trimesters, Total 853 patients from Antenatal clinic, period of three months from October to December 2021. Methods And Material: We collected the data from Central laboratory and performed Complete blood count and Solubility Test on Females within reproductive age group. We carried out a logistic regression analysis wit Statistical Analysis Used: h variable Sickle and explanatory and predictive analysis variables HB, Age, Platelet and total count. 0.12% of pati Result- ent was missing (1/853) with Negative 740/853 (87%) and Positive cases 112//853(13%), mean Hb (9.72± 1.59) P < 0.001 with Welch double test. Age in sickle positive cases had Mean (24.1± 3.45) with P= 0.58, Odds ratio 0.989 and P= 0.68. Mean Age and Hb display a Nonlinear Relationship with p= 0.23. Age and Platelet count have shown a linear Correlation with p=0.021, Age and total count have also showed signicant correlation with P=< 0.001. In this analysis we Conclusionfound that using a Method having fast accessibility and having good time approval test result like solubility gives a preliminary outlook towards SCD, though the test is having few limitations in differentiating Sickle cell trait and disease.