Affiliation:
1. Assistant Professor, Department of Pathology Madurai Medical College.
2. Assistant Professor,department of Pathology Madurai Medical College.
Abstract
BACKGROUND: Helicobacter pylori plays an important role in the causation of numerous benign, premalignant and
malignant lesions of Gastrointestinal tract which include peptic ulcer, gastritis, intestinal metaplasia, gastric
adenocarcinoma and Mucosa – associated lymphoid tissue lymphoma. In histopathological sections Helicobacter pylori can be identied by
various staining methods like Hematoxylin and Eosin (H&E), Toluidine blue, Modied Giemsa, Alcianyellow –Toluidine blue, Warthin-starry,
Modied Genta and Immunohistochemistry.
AIM OF THE STUDY: The aim of the present study is to compare the efcacy of Hematoxylin and Eosin, Giemsa , Toluidine blue and
Immunohistochemistry for the detection of H.pylori in cases of gastric adenocarcinoma.
MATERIALS AND METHODS: A total of 30 cases of gastric adenocarcinoma were randomly selected for this Retrospective study conducted
in Department of Pathology and all the four stains were applied and Immunohistochemical study using Helicobacter pylori polyclonal antibody
were also done in all cases.
RESULTS: Helicobacter pylori infection showed an overall prevalence rate of about 56.6%. When compared with Immunohistochemistry,
Sensitivity and specicity of Hematoxylin and Eosin was 41.18% and 100% respectively. Giemsa showed sensitivity of about 88.23% and
specicity of about 100%. Toluidine blue showed sensitivity of about 76.47% and specicity of about 100%.
CONCLUSION: Hence in the present study, Immunohistochemistry carries the highest level of sensitivity in the detection of Helicobacter
Pylori. Giemsa was more reliable and cost effective stain when compared with Hematoxylin & Eosin, Toluidine blue and
immunohistochemistry. However, Immunohistochemistry carries the highest level of sensitivity especially when the density of organism is low
and in clinically suspected cases of Helicobacter Pylori with negative Giemsa staining.
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