“IN VITRO STUDY OF HOMOEOPATHIC MEDICINE SARSAPARILLA Q, 6C, 12C, 30C, 200C, 1M AS AN INHIBITOR OF CALCIUM OXALATE AND CALCIUMPHOSPHATE CRYSTALLISATION.”-Reference-Cited by-同舟云学术

“IN VITRO STUDY OF HOMOEOPATHIC MEDICINE SARSAPARILLA Q, 6C, 12C, 30C, 200C, 1M AS AN INHIBITOR OF CALCIUM OXALATE AND CALCIUMPHOSPHATE CRYSTALLISATION.”

Published:2021-09-01 Issue: Volume: Page:73-76
ISSN:
Container-title:INTERNATIONAL JOURNAL OF SCIENTIFIC RESEARCH
language:en
Short-container-title:ijsr

Author:

R. Patel Mugdhakumari¹,A. Khan Tanveer²,Namdeo Ajay³,H. Shinde Chetan⁴

Affiliation:

1. Post graduate scholar, Department of Homeopathic Pharmacy, Bharati Vidyapeeth (Deemed to be University), Homoeopathic Medical College& Hospital, Pune, India, 411043.

2. I/C Principal, Bharati Vidyapeeth (Deemed to be University), Homoeopathic Medical College & Hospital, Pune, India, 411043.

3. Associate Professor, Department of Pharmacognosy, Bharati Vidyapeeth (Deemed to be University), Poona college of Pharmacy, Pune, India.

4. Professor, Department of Homoeopathic Pharmacy, Bharati Vidyapeeth (Deemed to be University), Homeopathic Medical College& Hospital, Pune, India, 411043.

Abstract

Background: Around 12% of world population has been infected by renal stone disease which has multiphase etiological factors with high recurrence rate. Thus, in order to reduce the recurrence rate, use of homoeopathic intervention can be the most effective alternative option and Sarsaparilla is one of the frequently prescribed medicines by homoeopath in renal stones. Aim: To nd out the inhibitory action of homoeopathic medicine Sarsaparilla Q, 6C, 12C, 30C, 200C, 1M on calcium oxalate and calcium phosphate crystallization by in vitro study. Methodology: In vitro crystallisation of calcium oxalate and calcium phosphate was carried out to evaluate effectiveness of Homoeopathic Medicine Sarsaparilla as an inhibitor. Calcium Oxalate crystallisation assay was experimented by using JASCO-UV/VISIBLE-630 Spectrophotometer. Slope of Nucleation and Aggregation phases is calculated using linear regression analysis and percentage of inhibition was calculated. Calcium phosphate crystallisation inhibition by Sarsaparilla was calculated by measuring concentration of calcium and phosphate ions, using Digital Photo colorimeter by Trinder and Gomeri method respectively. Antioxidant activity of Sarsaparilla Mother Tincture and potencies was measured by DPPH free radical scavenging assay by spectrophotometer. Result: Maximum inhibition of calcium oxalate crystallisation has been noticed in Sarsaparilla Mother Tincture and 1M potency. While Sarsaparilla 30C showing maximum inhibition of Calcium Phosphate crystallisation. Sarsaparilla mother tincture was able to decolorize during DPPH assay in which Inhibition % was decreased as potency increased (Q>30C>1M). Conclusion: The present in vitro study has shown potential role of Homoeopathic medicine Sarsaparilla as an inhibitor of calcium oxalate and calcium phosphate crystallisation.

Publisher

World Wide Journals

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