Affiliation:
1. Assistant Professor, Department of Microbiology, Rajiv Gandhi Medical College, Kalwa, Maharashtra, India.
2. Consultant Pathologist SRL Diagnostics Mumbai.
Abstract
Background: The non fermenter gram negative bacilli (NFGNB) are primarily opportunistic pathogens and have
emerged as an important cause of health care associated infections. The Methods: non fermenter organisms were isolated
from various clinical specimens in a microbiology laboratory of a tertiary care hospital and were identied by the conventional bacteriological
identication methods. Susceptibility testing was performed by methods as recommended by Clinical Laboratory Standard Institute (CLSI).
Results: A total of 10,456 clinical specimens were processed, of which 442 (4.23%) non fermenter spp. were isolated. Most common infection
caused by non fermenters was abscess. Maximum numbers of isolate (52.94%) were of Pseudomonas aeruginosa, followed by Acinetobacter
baumannii (42.76%). Other species isolated were Acinetobacter calcoaceticus (1.81%), Pseudomonas putida (1.13%), Acinetobacter lwofi
(0.68%), Pseudomonas stutzeri (0.45%), Stenotrophomonas maltophilia (0.23%). The isolation rate of non fermenters from ICU was much
higher compared to general ward. High antibiotic resistance was noted for commonly used antibiotics like cephalosporins, quinolones,
aminoglycosides. A total 58.60% of multi drug resistant (MDR) non fermenter isolates were obtained. The isolation rate of MDR isolates of non
fermenters was more from ICU compared to general ward. Nosocomial infections res Interpretation and Conclusion: istant to most
antimicrobials caused by non fermenter organisms have emerged, especially in ICU. Early identication and continued surveillance will help to
prevent their spread in hospital environment.
Reference16 articles.
1. Bergogne-Berezin E, Towner KJ. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical and epidemiological features. Clin Microbiol Rev 1996; 9:148–65.
2. Forbes B, Sahm D, Weissfeld A. Pseudomonas, Burkholderia, and similar organisms. In: Bailey and Scott's Diagnostic microbiology.12th ed St. Louis, Missouri: Elsevier 2007:340-9.
3. Juyal D, Prakash R, Shanakarnarayan S, Sharma M, Negi V and Sharma N. Prevalence of non-fermenting gram negative bacilli and their in vitro susceptibility pattern in a tertiary care hospital of Uttarakhand: A study from foothills of Himalayas. Saudi J for Health Sciences 2013; 2(2):108-112.
4. Collee JG, Marr W. Specimen collection, culture containers and media. In: Collee JG, Fraser AG, Marmion BP, Simmons A. Mackie & McCartney practical medical microbiology. 14th Ed. Edinburgh: Churchill Livingstone 2006: 95-112.
5. Duguid JP. Staining methods. In: Collee GJ, Fraser AG, Marmion BP, Simmons A. Mackie & McCartney practical medical microbiology. 14th ed. Edinburgh: Churchill Livingstone 2006:793-812.