Evaluation of Different DNA İsolation Techniques in Honey, Pollen And Propolis Samples Belonging to Çankırı Province

Abstract

Honey is a natural and complex nutrient produced by collecting and processing secretions obtained from insects that feed on nectar or plant extract by bees. Honey, which is one of the most consumed natural foods, has many benefits in terms of human health. One of the most effective ways to access this information is to isolate the DNA found in honey by analyzing some of the specific gene sequences it contains. However, due to the inhibitors it contains, which are quite viscous in nature, there are a number of problems and limitations in the analysis of DNA in honey. In present study, it was aimed to compare 3 different isolation techniques, CTAB, TRIzol methods and DNeasy® Plant Pro Kit in honey, pollen and propolis samples and to recommend the most appropriate method for these samples. As a result of the spectrophotometric method and gel analyzes to image the genomic DNA, DNA isolation from propolis samples could not be performed with CTAB and DNeasy® Plant Pro Kit methods, and DNA isolation from honey sample could not be performed in TRIzol method. The TRIzol method was successful compared to the other two methods for the propolis sample. Especially for the propolis sample with a very dense chemical structure, the TRIzol method was studied for the first time and yield was obtained. The data obtained as a result of this study support the implementation of such applications for other types of honey and honey-based products produced in our country and thus the raising of the quality standards of honey, which has an important place in the economy of our country.