Affiliation:
1. Kağızman Devlet Hastanesi
2. ATATÜRK ÜNİVERSİTESİ
Abstract
Carbonic anhydrases (CAs) are a metalloenzyme that contains zinc ions in their active sites, and they reversibly catalyze the reactions of hydration of CO2 and dehydration of HCO3-in the living. In this study, human carbonic anhydrase II (hCAII) isoenzyme was purified from human erythrocytes by affinity column (Sepharose 4B-L-tyrosine-sulfanilamide). The inhibitory effect of artificial tear drops on hCAII isoenzyme was investigated. IC50 values were calculated as 1.01 µL for sodium hyaluronate, 140.06 µL for polyvinyl alcohol+povidone, and 49.51 for polyethylene glycol+propylene glycol. The Ki value for sodium hyaluronate was found as 0.339±0.121 mM. According to these results, sodium hyaluronate was the most effective inhibitor in terms of application volume (microliter) among the three drugs examined.
Reference31 articles.
1. Bromberg, B.B., Welch, M.H., Beuerman, R.W., Chew, S-J., Thompson, H.W., Ramage, D., Githens, S., (1993). Histochemical distribution of carbonic anhydrase in rat and rabbit lacrimal gland. Investigative Ophthalmology & Visual Science, 34: 339-348
2. Bron, A. J., Tomlinson, A., Foulks, G. N., Pepose, J. S., Baudouin, C., Geerling, G., ... & Lemp, M. A. (2014). Rethinng dry eye disease: a perspective on clinical implications. The Ocular Surface, 12(2), S1-S31.
3. Candia, O.A., (1996). A novel system to measure labelled CO2 and HCO3 fluxes across epithelia: corneal epithelium as model tissue. Experimental Eye Research, 63:137–149
4. Carradori S, Mollica A, De Monte C, Ganese A, Supuran CT. (2015) Nitric oxide donors and selective carbonic anhydrase inhibitors: a dual pharmacological approach for the treatment of glaucoma, cancer and osteoporosis. Molecules, 20:5667-5679.
5. Cerdà‐Costa, N., & Xavier Gomis‐Rüth, F., (2014). Architecture and function of metallopeptidase catalytic domains. Protein Science, 23(2), 123-144.