Characterisation of Trichoderma spp. and Assessment as Biocontrol Using Dual Culture Assay Against Fungi Associated with Black Pepper (Piper nigrum L.) Diseases in Sarawak

Author:

KWAN YEE MIN,WILSON JOY FRANCO

Abstract

Black pepper (Piper nigrum L.) is one of the most widely used spices in food, beverage, cosmetics, and medicine. Black pepper production has suffered from various fungal diseases. Microbial biological control is an essential part of integrated disease management to reduce the heavy reliance on chemical fungicides. Trichoderma fungi comprise a large group of rhizocompetent filamentous fungi widely used in the biocontrol of plant pathogens. Three field surveys conducted on five black pepper farms in Belaga, Sarawak, identified three fungal diseases: yellowing, black berry, and foot rot. Based on the morphological and molecular characterisation, the identified fungal causal agents were Fusarium solani (yellowing disease), Colletotrichum gloeosporioides (black berry disease), and Phytophthora palmivora (foot rot disease). Twenty isolates of Trichoderma spp. were isolated from secondary forest and Biopark in Bintulu, Sarawak. Trichoderma isolates were characterised based on the morphological characteristics and molecular phylogenetic analysis using the rDNA internal transcribed spacer (ITS) region. Trichoderma isolates were separated into five distinct species, namely T. harzianum, T. virens, T. brevicompactum, T. tawa, and telomorphic Hypocrea lixii. Among the Trichoderma fungi, T. harzianum was the most frequently (65%) isolated species. Trichoderma harzianum (Isolates of TJ9, 10, and 16) showed antagonistic and inhibitory effects by 61 to 70% on in vitro mycelial growth against three common fungal pathogens of black pepper, P. palmivora, C. gloeosporioides, and F. solani. This study highlights the potential of using native Trichoderma fungi as biocontrol agents in the black pepper integrated disease management program.

Publisher

UNIMAS Publisher

Subject

Water Science and Technology,Molecular Biology,Animal Science and Zoology,Ecology,Biochemistry,Ecology, Evolution, Behavior and Systematics,Biotechnology,Forestry

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3