Effect of Media and Temperature on Growth and Preliminary Detection of Ligninolytic and Cellulolysic Activity of Trametes spp.

Abstract

Effects of media and temperatures on growths of Trametes cervina (Schwein.) Bres., T. ljubarski Pilat, T. orientalis (Yasuda) Imazeki and T. velutina (Pers.) G. Cunn were studied. There was significant different of effect of media on growth of all the Trametes isolates. All of the four Trametes isolates were able to grow on Malt Extract Agar (MEA), Potato Dextrose Agar (PDA), Yeast Malt Agar (YMA) and Corn Meal Agar (CMA), however, no growth of T. cervina was seen on Czapek Dox Agar (CDA). Colony growth rates varied depending on the media and isolate of the fungi. T. orientalis showed the fastest growth while T. cervina showed the slowest growth on all the tested media. Although, T. ljubarski, T. orientalis and T. velutina can grow on CDA, the formation of mycelia was sparsely. There were significant differences of effect of temperature on growth of the Trametes isolates. T. orientalis and T. velutina, both, can grow at temperature up to 40oC, T. ljubarski can growth at temperature up to 35oC while T. cervina at temperature up to 30oC only. The optimum growth temperature for, both, T. orientalis and T. velutina was at 30oC, while for isolate T. ljubarski at 35oC and T. cervina at 25oC. All of the four Trametes isolates showed excellent cellulosic activity, indicated by the formation of clearing zone in the test media. The diameters of the degraded area formed by all of the Trametes isolates were more than 8 cm. T. orientalis and T. ljubarski degraded lignin better than T. cervina or T. velutina