Studies of Insulin Binding in Children Using Human Erythrocytes in Small Amounts of Blood

Author:

Kappy Michael S1,Plotnick Leslie1

Affiliation:

1. Endocrinology Division, Department of Pediatrics, The Johns Hopkins Hospital Baltimore, Maryland 21205

Abstract

We have demonstrated specific insulin binding by the erythrocytes (RBCs) of children. Complete binding studies were done using as little as 5 ml of blood. The receptors exhibited competition-inhibition curves and nonlinear Scatchard plots similar to those reported for insulin target tissues, such as the hepatocyte and the adipocyte. Compared with those from adults, the RBCs from children had significantly greater numbers of insulin receptors per cell (P < 0.05). The total insulin bound by the RBCs from both children and adults, however, was not different over the physiologic range of insulin concentrations. Cord blood RBCs showed greater numbers of receptors per cell than did those from either children or adults; however, the affinity for insulin was similar in both groups. The total amount of insulin bound by cord blood was significantly greater than that in either children (P < 0.01) or adults (P < 0.05) over the physiologic range of insulin concentrations. The method used to measure insulin binding by erythrocytes had relatively little intra- and interassay variability, and there was little diurnal variation in binding. Storage of heparinized blood at 4°C for 24-36h had no effect on insulin binding by the RBCs. We conclude that the measurement of insulin binding by RBCs from small volumes of blood may be particularly useful in the study of infants and children with disorders of carbohydrate metabolism to elucidate the role, if any, of abnormal receptor function in their condition.

Publisher

American Diabetes Association

Subject

Endocrinology, Diabetes and Metabolism,Internal Medicine

Cited by 8 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Reticulocytes and Insulin Binding to Erythrocytes1;Acta Medica Scandinavica;2009-04-24

2. Distribution of insulin receptors in human erythrocyte membranes. Insulin binding to sealed right-side-out and inside-out human erythrocyte vesicles;Biochimica et Biophysica Acta (BBA) - Biomembranes;1984-08

3. Developmental changes in insulin receptors of pig red blood cells;Archives of Biochemistry and Biophysics;1984-07

4. Binding of tyrosine-A-14(125I)-monoiodoinsulin to human erythrocytes;Scandinavian Journal of Clinical and Laboratory Investigation;1984-01

5. Isolation and characterization of human erythrocyte insulin receptors.;Journal of Biological Chemistry;1983-04

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