Heterogeneity of Circulating Human C-peptide

Author:

Kuzuya Hideshi1,Blix Petra M2,Horwitz David L2,Rubenstein Arthur H2,Steiner Donald F2,Faber Ole K3,Binder Christian3

Affiliation:

1. Department of Medicine, Kyoto University Kyoto, Japan

2. Departments of Medicine and Biochemistry, University of Chicago Klampenborg, Denmark

3. Hvidøre Hospital Klampenborg, Denmark

Abstract

Values reported for serum C-peptide immunoreactivity in healthy individuals vary considerably. To assess factors that contribute to this finding, three human C-peptide assay systems were developed utilizing three distinct antisera that react differently with various C-peptide fragments. Preparations of natural pancreatic and synthetic human C-peptide standards were compared immunologically in these systems. The curves of the natural C-peptide and the synthetic preparations were not identical. The relative immunoreactivity of each standard varied, depending on the particular antiserum used. Serum C-peptide concentrations varied when measured in the different assay systems. Furthermore, the results of dilution and recovery tests and stability of the C-peptide during storage showed differences among the three assays. Gel filtration of serum indicated heterogeneity within the major C-peptide peak, and, in addition, a smaller peak of lower molecular weight material was present in some samples. Although degradation of serum C-peptide may occur during storage, fragments of C-peptide may also be secreted or arise during in-vivo metabolism. Thus, the present studies indicate the need for careful standardization and checking of each particular assay system before its use in clinical studies.

Publisher

American Diabetes Association

Subject

Endocrinology, Diabetes and Metabolism,Internal Medicine

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