Preliminary Studies on the Demonstration of Insulin in the Islets by the Fluorescent Antibody Technic

Abstract

Since Moloney and Coval1 have demonstrated that antibodies to insulin can be produced in the guinea pig, it seemed feasible that these antibodies could be labeled with fluorescein isocyanate by the method of Coons and Kaplan2 and used as an immunochemical stain for insulin. The results obtained from the application of these labeled antibodies to frozen sections of pancreas are reported in this paper.