Cell-Free Synthesis of Rat Insulin-like Growth Factor II-Reference-Cited by-同舟云学术

Cell-Free Synthesis of Rat Insulin-like Growth Factor II

Published:1982-07-01 Issue:7 Volume:31 Page:656-658
ISSN:0012-1797
Container-title:Diabetes
language:en
Short-container-title:

Author:

Acquaviva Angela Maria¹,Bruni Carmelo Bruno¹,Nissley S Peter²,Rechler Matthew M¹

Affiliation:

1. Section on Biochemistry of Cell Regulation, Laboratory of Biochemical Pharmacology, National. Institute of Arthritis, Diabetes, and Digestive and Kidney Diseases Bethesda, Maryland

2. Endocrine Section, Metabolism Branch, National Cancer Institute, National Institutes of Health Bethesda, Maryland

Abstract

Total RNA extracted from a rat liver cell line (BRL 3A) that synthesizes rat insulin-like growth factor II (IGF-II) was translated in a reticulocyte lysate cell-free system. Incubation of the translation products with antisera to mature rat IGF-II (Mr 8700) selectively immunoprecipitated a Mr 21,600 protein. We propose that this protein represents pre-pro-IGF-II.

Publisher

American Diabetes Association

Subject

Endocrinology, Diabetes and Metabolism,Internal Medicine

Link

https://journals.org/diabetes/diabetes/article-pdf/31/7/656/352335/31-7-656.pdf

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