Affiliation:
1. Departments of Medicine and Biochemistry, Louisiana State University School of Medicine New Orleans, Louisiana
Abstract
A study of connective tissue components from skin of rats with induced diabetes and hyperinsulinism was performed. Diabetes was induced in rats by a single injection of streptozotocin (50 to 75 mg./kg. body weight) and hyperinsulinism by daily injection of NPH insulin (½ to 2½ U.). Serum was analyzed for protein-bound carbohydrates and free sugars. A decrease in serum protein-bound sugars was observed in the diabetic animals. These slightly increased in hyperinsulin state. Acid mucopolysaccharides (MPS), glycoproteins (GP) and collagen were isolated from skin of experimental animals and compared with the controls. The contents of total MPS and GP decreased in diabetic skin; these increased in hyperinsulin state, similar to that observed in human diabetes. Diabetic rat skin had more soluble collagen, while less soluble collagen was observed in hyperinsulin state. It is suggested from these observations that insulin is a driving force toward the synthesis and accumulation of these compounds in connective tissue in experimental hyperinsulinism and, perhaps, in human diabetes with prolonged insulin treatment.
Publisher
American Diabetes Association
Subject
Endocrinology, Diabetes and Metabolism,Internal Medicine
Cited by
38 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献