Affiliation:
1. Department of Internal Medicine and Center for Human Nutrition, Washington University School of Medicine, St. Louis, Missouri
2. Saint Luke’s Hospital, Kansas City, Missouri
Abstract
We evaluated the validity of using a single fatty acid tracer to assess total plasma long-chain free fatty acid (FFA) kinetics and the relationship between the rate of appearance (Ra) of fatty acids in plasma and the fatty acid composition of adipose tissue triglyceride (TG). A mixture of [13C]-labeled myristate, palmitate, stearate, oleate, and linoleate was infused in healthy men during basal conditions and during conditions that stimulate (epinephrine infusion) and inhibit (insulin infusion) lipolysis of adipose tissue TGs. Calculated total FFA, Ra based on palmitate, oleate, or linoleate tracers, was within 15% of the measured sum of the individual fatty acid Ra under all conditions, whereas stearate and myristate tracers consistently underestimated and overestimated total FFA Ra, respectively. The fatty acid Ra profile closely matched the fatty acid profile of subcutaneous adipose tissue TGs during epinephrine infusion, but not during basal conditions and insulin infusion. Our data support the common practice of using labeled palmitate or oleate as fatty acid tracers for assessing total plasma FFA kinetics and suggest that a source of lipids other than adipose tissue TG release fatty acids into the systemic circulation.
Publisher
American Diabetes Association
Subject
Endocrinology, Diabetes and Metabolism,Internal Medicine
Reference37 articles.
1. Rebrin K, Steil GM, Getty L, Bergman RN: Free fatty acid as a link in the regulation of hepatic glucose output by peripheral insulin. Diabetes 44:1038–1045,1995
2. Boden G, Chen X, Ruiz J, White JV, Rossetti L: Mechanisms of fatty acid-induced inhibition of glucose uptake. J Clin Invest 93:2438–2446,1994
3. Lewis GF: Fatty acid regulation of very low density lipoprotein production. Curr Opin Lipidol 8:146–156,1997
4. Wolfe RR: Radioactive and Stable Isotope Tracers in Biomedicine: Principles and Practice of Kinetic Analysis. New York, Wiley-Liss,1992
5. Racette SB, Horowitz JF, Mittendorfer B, Klein S: Racial differences in lipid metabolism in women with abdominal obesity. Am J Physiol Regul Integr Comp Physiol 279:R944–R950,2000
Cited by
95 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献